Serrano R, Bernal D, Simon E, Arino J. (2004) Copper and iron are the limiting factors for growth of the yeast Saccharomyces cerevisiae in an alkaline environment. J. Biol. Chem. 279, 19698-19704.

 

 

 

Table 1.- Mutations that results in growth defect under alkaline conditions. Numbers in parentheses indicates the level of alkaline sensitivity (being 1 the highest sensitivity). Genes in bold type correspond to those identify in a recent report (11) as conferring a fitness defect when deleted. For details see the main text.

 

Function

Alkaline pH sensitive mutants

 

 

Vacuole organization and biogenesis

 

CUP5 (1), TFP1 (1), VMA2 (1), VMA22 (1), VMA4 (1), VMA5 (1), VMA6 (1), VMA7 (1), CWH36 (1), VMA21 (2), PEP3 (2), PEP5 (2), PPA1 (2), TFP3 (2), VMA13 (2), VPS16 (2), VPS33 (2), VPS34 (2), ARP5 (3), VPS15 (3), FAB1 (4), PEP7 (4), VMA8 (4), VPS65 (5)

Transport in other organelles

 

GCS1 (3), SEC22 (3), DRS2 (3), VPS20 (4), GLO3 (4), COG8 (4), SYS1 (4)

Metal ion homeostasis

 

CTR1 (1), RCS1 (1), SOD1 (1), LYS7 (2), SOD2 (2), CCC2 (4), FET3 (4), PMR1 (4)

Metabolism

Aminoacid metabolism

Lipid metabolism

Phosphate metabolism

 

GLY1 (1), PHO2 (1), PRO1 (1), CYS3 (3), ARO2 (4), ILV1 (4), TYR1 (5)

ERG2 (2), ERG6 (2), ARV1 (3), BTS1 (3), AGP2 (4), DAP1 (4), FEN1 (4), MGA2 (4)

PHO4 (2), PHO81 (2), PHO85 (3)

Protein biosynthesis

 

TEF4 (3), FES1 (4), HCR1 (4), RPL13B (4), RPL27A (4)

Cell polarity, cell wall organization and biogenesis

 

RHO4 (1), BUD25 (1), BEM1 (2), GAS1 (2), SLT2 (2), BCK1 (3), BEM4 (4), CNB1 (5)

Chromatin modification, architecture & transcription

 

SWI3 (1), BUR2 (3), DAL81 (3), GCN5 (3), HTZ1 (3), REF2 (3), REG1 (3), RPB9 (3), SPT20 (3), SSN8 (3), UME6 (3), ADA2 (4), KEM1 (4), MED2 (4), SPC72 (4), SRB5 (4), SWI4 (4), TOP1 (4), MRS1 (5)

Others

 

PHO86 (1), KEX2 (1), RIB4 (1), DIA2 (2), SPS1 (2), FYV6 (3), MAP1 (3), TPD3 (3), ADE12 (4), ATP1 (4), ATP11 (4), NEM1 (4), PTC1 (4), URE2 (4), ZWF1 (4), COX16 (5), RIB1 (5), SCP160 (5)

Unknown

 

YEL045C (1), YKL118W (1), YOR331C (1), YJL175W (3), HUR1 (4), ILM1 (4), PKR1 (4), YLR358C (4), YJR018W (5), YOR251C (5), YOR305W (5)