Table 2. Classification into functional families of ORFs whose transcripts are induced at least 2-fold after alkaline pH stress. The asterisks denote that the intensity of the hybridisation signal was out of the linear range of detection and, therefore, induction might be stronger than that presented here. The «Time» column indicates the kinetic of response. Genes showing the highest induction after 5 min at alkaline pH are denoted by «E» (Early). «I» (Intermediate) indicates a peak at 25 min and «L» (Late) corresponds to genes that shows the highest induction after 45 min at alkaline pH. Functional categories were assigned based on information provided by MIPS (Mewes et al., 1999). Boldface indicates genes identified as responsive to a wide variety of stresses (Gasch et al., 2000; Causton et al., 2001)
|
ORF |
Gene |
-Fold |
Time |
Function |
Carbohydrate Metabolism / Energy
YOR374W |
ALD4 |
6.8 |
E |
Mitochondrial aldehyde dehydrogenase |
|
YPL061W |
ALD6 |
5.0 (*) |
I |
Acetaldehyde dehydrogenase |
|
YOR095C |
RKI1 |
3.3 |
I |
Ribose-5-phosphate ketol-isomerase |
|
YEL071W |
DLD3 |
3.9 (*) |
I |
D-lactate dehydrogenase |
|
YIL162W |
SUC2 |
4.6 |
E |
Sucrose utilization |
|
YDR178W |
SDH4 |
2.4 |
E |
Subunit succinate dehydrogenase complex |
|
YCR005C |
CIT2 |
4.1 |
I |
Peroxisomal citrate synthase |
Phosphate transport / metabolism
|
YBR296C |
PHO89 |
3.2 |
E |
High-affinity Na+-phosphate symporter |
|
YHR215W |
PHO12 |
2.2 |
L |
Inducible, secreted acid phosphatase |
|
YML123C |
PHO84 |
12.7 |
L |
H+/phosphate permease |
|
YJL012C |
VTC4 |
2.9 |
I |
Putative polyphosphate synthetase |
|
YPL019C |
VTC3 |
2.2 |
L |
Putative polyphosphate synthetase |
|
YER072W |
VTC1 |
2.5 |
L |
Putative polyphosphate synthetase |
Other metabolism
|
YLR303W |
MET17 |
2.2 |
I |
Methionine biosynthesis |
|
YKL001C |
MET14 |
4.2 |
I |
Sulfate assimilation |
YGL037C |
PNC1 |
2.3 |
E |
Pyridine nucleotide cycle |
|
YML106W |
URA5 |
5.0 |
I |
Pyrimidine biosynthesis |
|
YPL057C |
SUR1 |
2.2 |
I |
Sphingolipid metabolism |
|
YLR056W |
ERG3 |
2.1 |
L |
Sterol metabolism |
|
YOL165C |
AAD15 |
2.8 |
I |
Hypothetical aryl-alcohol dehydrogenase |
|
YCR107W |
AAD3 |
2.5 |
I |
Putative aryl-alcohol dehydrogenase |
|
YFL056C |
AAD6 |
2.5 |
I |
Probable aryl alcohol dehydrogenase |
Ion transport & homeostasis / Other transports
|
YBR158W |
CST13 |
4.2 |
I |
Cu2+ ion homeostasis (putative |
|
YLR214W |
FRE1 |
7.4 |
I |
Iron and copper homeostasis |
|
YOR382W |
FIT2 |
6.6 |
L |
Possibly involved in iron uptake |
|
YPL135W |
ISU1 |
2.3 |
I |
Iron homoestasis |
|
YEL065W |
ARN3 |
5.4 |
I |
Ferrioxamine B permease |
|
YHL040C |
ARN1 |
2.4 |
L |
Transport of ferrichromes. |
|
YHL047C |
ARN2 |
3.9 |
L |
Triacetylfusarinine C transporter |
|
YMR058W |
FET3 |
2.6 |
L |
Cell surface ferroxidase |
|
YPR124W |
CTR1 |
2.5 |
I |
Required for high-affinity uptake of copper |
|
YDR038C |
ENA5 |
2.5 |
E |
Na(+) ATPase |
|
YDR040C |
ENA1 |
2.0 |
E |
Na(+) ATPase |
|
YCR011C |
ADP1 |
2.1 |
I |
Putative ATP-dependent permease |
|
YDR011W |
SNQ2 |
3.1 |
I |
Putative ATP-dependent permease |
|
YGL077C |
HNM1 |
2.2 |
E |
Choline permease |
|
YGR121C |
MEP1 |
2.0 |
E |
Ammonia permease |
|
YHR092C |
HXT4 |
2.3 |
E |
Moderate- to low-affinity hexose transporter |
|
YIL170W |
HXT12 |
2.7 |
E |
Hexose transporter |
Transcription & RNA processing
|
YBR112C |
CYC8 |
2.9 |
I |
General repressor |
|
YBR154C |
RPB5 |
7.2 |
I |
Shared subunit of RNA pol. I. II. III |
|
YCL066W |
HMLa1 |
2.3 |
I |
Activates alpha-specific genes |
|
YJR063W |
RPA12 |
2.1 |
I |
RNA polymerase I subunit |
|
YKR025W |
RPC37 |
2.0 |
I |
RNA polymerase III 37 kDa subunit |
|
YPR110C |
RPC40 |
2.3 |
I |
Shared subunit of RNA pol. I and III |
|
YLR136C |
TIS11 |
4.6 |
I |
Pol II transcription |
|
YDR395W |
SXM1 |
2.3 |
I |
mRNA export. nuclear protein targeting |
|
YHR170W |
NMD3 |
2.2 |
I |
mRNA decay |
|
YKL009W |
MRT4 |
2.6 |
I |
mRNA decay |
|
YOL123W |
HRP1 |
2.0 |
E |
Nuclear polyA RNA-binding protein |
|
YDR083W |
RRP8 |
2.9 |
I |
Ribosomal RNA processing |
|
YHR169W |
DBP8 |
2.1 |
I |
RNA helicase |
|
YJL116C |
NCA3 |
9.7 |
I |
RNA processing/modification |
|
YDR087C |
RRP1 |
3.8 |
I |
rRNA processing |
|
YJL033W |
HCA4 |
3.5 |
I |
rRNA processing |
|
YOL142W |
RRP40 |
2.2 |
I |
rRNA processing |
|
YDL051W |
LHP1 |
2.3 |
I |
tRNA processing |
Stress response
YMR174C |
PAI3 |
4.5 |
I |
Osmotic stress response |
YJR104C |
SOD1 |
2.1 |
I |
Copper-zinc superoxide dismutase |
YKR066C |
CCP1 |
2.6 |
I |
Cytochrome-c peroxidase |
|
YCR021C |
HSP30 |
2.0 |
L |
Heat shock protein |
YDR513W |
GRX2 |
2.3 |
I |
Glutaredoxin |
|
YDR353W |
TRR1 |
4.6 (*) |
I |
Thioredoxin reductase |
|
YBR111C |
YSA1 |
2.1 |
I |
Nucleoside diphosphate-sugar hydrolase |
|
YMR175W |
SIP18 |
2.1 |
I |
Induced by osmotic stress |
Protein degradation / synthesis / modification
|
YER012W |
PRE1 |
2.1 |
I |
20S proteasome subunit C11(beta4) |
|
YGR135W |
PRE9 |
2.2 |
I |
20S proteasome subunit Y13 (alpha3) |
|
YLR120C |
YPS1 |
2.7 |
E |
GPI-anchored aspartyl protease |
YPL154C |
PEP4 |
2.1 |
E |
Vacuolar aspartyl protease |
|
YDR037W |
KRS1 |
2.2 |
I |
tRna synthetase. lysyl |
|
YDR041W |
RSM10 |
2.0 |
E |
Mitochondrial ribosomal small subunit |
|
YDR418W |
RPL12B |
2.2 (*) |
I |
Ribosomal protein L12B |
|
YNL002C |
RLP7 |
2.0 |
I |
Similarity to ribosomal proteins |
|
YOL127W |
RPL25 |
3.7 |
L |
Ribosomal protein L25 |
|
YMR060C |
TOM37 |
3.1 |
L |
Outer membrane translocase |
|
YBR110W |
ALG1 |
5.3 |
I |
Beta-1.4-mannosyltransferase |
|
YBR205W |
KTR3 |
3.0 |
I |
Putative alpha-1.2-mannosyltransferase |
|
YMR101C |
SRT1 |
2.0 |
L |
Cis-prenytransferase (putative) |
Other
|
YAL059W |
ECM1 |
2.0 |
I |
Cell wall biogenesis |
YGR008C |
STF2 |
2.3 |
E |
Cell wall maintenance |
|
YKL096W |
CWP1 |
2.2 |
E |
Mannoprotein of the cell wall |
|
YBR160W |
CDC28 |
2.4 |
I |
Cyclin-dependent protein kinase |
|
YNL307C |
MCK1 |
2.2 |
E |
Member of the GSK-3 family of protein kinases |
|
YOR242C |
SSP2 |
3.7 |
L |
Sporulation |
|
YCR052W |
RSC6 |
2.9 |
I |
Chromatin remodeling complex subunit |
|
YCR009C |
RVS161 |
2.1 |
I |
Actin-binding protein |
|
YBR179C |
FZO1 |
3.4 |
E |
Mitochondrial membrane GTPase |
|
YIR012W |
SQT1 |
2.5 |
I |
Ribosome biogenesis. putative |
YNL036W |
NCE103 |
7.6 |
E |
Secretion |
|
YBR109C |
CMD1 |
2.8 |
I |
Calmodulin. Ca+ binding |
YGR023W |
MTL1 |
2.3 |
L |
Morphogenesis |
|
YKL043W |
PHD1 |
3.1 |
E |
Transcription factor involved in filamentous growth |
Unknown
|
YAL049C |
|
2.6 |
I |
|
YAL053W |
|
2.0 |
L |
|
YBL108W |
|
3.2 |
L |
|
YBR004C |
|
2.2 |
E |
|
YBR207W |
FTH1 |
2.6 |
I |
YBR287W |
|
3.7 |
E |
|
YCL059C |
KRR1 |
2.3 |
I |
|
YCR054C |
CTR86 |
3.0 |
I |
|
YDL046W |
|
4.6 |
E |
|
YDL167C |
ARP1 |
2.6 |
I |
|
YDL213C |
|
3.7 |
I |
|
YDR161W |
TCI1 |
2.0 |
I |
|
YDR370C |
|
5.0 |
I |
|
YDR372C |
|
2.7 |
I |
YDR516C |
|
2.3 |
E |
|
YDR534C |
|
2.5 |
L |
YER150W |
SPI1 |
2.7 |
E |
|
YGL039W |
|
2.0 |
I |
|
YGL183C |
|
5.0 |
I |
|
YGR187C |
HGH1 |
2.0 |
I |
|
YGR211W |
ZPR1 |
2.0 |
I |
|
YGR213C |
RTA1 |
3.4 |
E |
|
YGR243W |
|
2.5 |
E |
YHL021C |
|
2.1 |
E |
|
YHR052W |
|
2.2 |
I |
YHR087W |
|
2.4 |
E |
YHR138C |
|
2.6 |
E |
|
YJL171C |
|
2.4 |
E |
|
YKL071W |
|
13.8 |
I |
|
YKL175W |
ZRT3 |
2.3 |
L |
|
YLR108C |
|
2.3 |
I |
|
YLR186W |
|
2.3 |
I |
YLR205C |
|
2.2 |
I |
YLR327C |
|
5.2 |
E |
|
YLR405W |
|
2.1 |
L |
|
YLR414C |
|
2.5 |
E |
|
YML058C-A |
|
2.1 |
L |
|
YML059C |
|
2.1 |
L |
YML128C |
MSC1 |
3.0 |
E |
YML131W |
|
2.9 |
I |
|
YMR031C |
|
2.9 |
E |
YMR173W |
DDR48 |
5.4 |
I |
YMR173W-A |
|
5.9 |
I |
|
YMR251W-A |
HOR7 |
9.2 |
E |
|
YMR304C-A |
|
2.3 |
E |
|
YMR316W |
DIA1 |
2.8 |
E |
|
YMR318C |
|
2.8 |
I |
|
YNL013C |
|
2.6 |
L |
YNL134C |
|
2.7 |
I |
|
YNL149C |
|
2.3 |
I |
YNL208W |
|
2.2 |
I |
|
YNL300W |
TOS6 |
2.2 |
L |
YOL150C |
|
3.0 |
I |
YOL151W |
GRE2 |
4.1 |
I |
|
YOR049C |
|
3.7 |
E |
YOR161C |
|
2.4 |
E |
|
YPL183C |
|
2.3 |
L |
|
|
|
|
|
|
|
|
|
|