{"id":76,"date":"2018-10-08T20:03:43","date_gmt":"2018-10-08T20:03:43","guid":{"rendered":"https:\/\/webs.uab.cat\/hrnases\/research\/"},"modified":"2026-02-12T19:10:45","modified_gmt":"2026-02-12T19:10:45","slug":"research","status":"publish","type":"page","link":"https:\/\/webs.uab.cat\/hrnases\/en\/research\/","title":{"rendered":"Publications"},"content":{"rendered":"

Selected publications<\/h2>\n

Anguita R, Li J, Boix E and Prats-Ejarque G A Novel Method to Monitor the Evolution of Antimicrobial Resistance in Acinetobacter baumannii Biofilms. IJMS<\/em>. 2026; 27, 1512<\/p>\n

Anguita R, Ali Y, Becknell B and Boix E* Ribonucleases of Mice and Men: Unveiling the Roles of the RNase A Superfamily in Host Defence J Immunol Res<\/em> 2025 10:2025 :3940139. DOI:\u00a0<\/span>10.1155\/jimr\/3940139<\/a><\/span><\/p>\n

Boix E, Li J. Ancestral ribonucleases back in motion for evolutionary-
\ndynamics guided protein design. Trends Biochem Sci<\/em>. 2024 Jun
\n15:S0968-0004(24)00148-8. DOI: <\/span> 10.1016\/j.tibs.2024.06.005\u00a0<\/a><\/span><\/p>\n

Li J, Kang X, Guidi I, Lu L, Fern\u00e1ndez-Mill\u00e1n P, Prats-Ejarque G, Boix E.
\nStructural determinants for tRNA selective cleavage by RNase 2\/EDN. Structure<\/em>.
\n2024 , S0969-2126(23)00453-7. DOI: <\/span> 10.1016\/j.str.2023.12.012 <\/a><\/span><\/p>\n

Cortado H, Kercsmar M, Li B, Vasquez-Martinez G, …Boix E, Zepeda-Orozco D, Jackson AR, Spencer JD, Ruiz-Rosado JD, Becknell B. Murine Ribonuclease 6 Limits Bacterial Dissemination During Experimental Urinary Tract Infection. J Innate Immun.<\/em> 2024 May 14. DOI:\u00a0<\/span>10.1159\/000539177<\/a><\/span><\/p>\n

Anguita R, Prats-Ejarque G, Moussaoui M, Becknell B, Boix E. A Common
\nPolymorphism in RNASE6 Impacts Its Antimicrobial Activity toward
\nUropathogenic Escherichia coli. Int J Mol Sci<\/em>. 2024 , 25(1):604. doi:<\/a>
\n10.3390\/ijms25010604.<\/a><\/p>\n

Rodriguez-Carlos A, Anguita R, Jacobo-Delgado YM, Serrano CJ, Santos-Mena A; De Jesus Gonzalez, LA, Boix E<\/strong>*, Rivas-Santiago B. Drug repurposing identifies histone deacetylase inhibitors that promote innate immunity in non tuberculous mycobacterial infection Can J Microbiol.<\/em> 2024 Jul 1;70(7):252-261.<\/p>\n

Ruiz-Rosado JD, Cortado H, …, Boix E, Jackson AR, Spencer JD, Becknell B.
\nHuman Ribonuclease 6 Has a Protective Role during Experimental Urinary Tract
\nInfection. J Innate Immun<\/em>. 2023;15(1):865-875. DOI: <\/span> 10.1159\/000534736 <\/a><\/span><\/p>\n

Abeng\u00f3zar M\u00c1, Fern\u00e1ndez-Reyes M, Salazar VA, Torrent M, de la Torre BG, Andreu D, Boix E, Rivas L. Essential Role of Enzymatic Activity in the Leishmanicidal Mechanism of the Eosinophil Cationic Protein (RNase 3). ACS Infect Dis<\/em>. (2022) 8(7):1207-1217. https:\/\/pubs.acs.org\/doi\/10.1021\/acsinfecdis.1c00537<\/a><\/p>\n

Fern\u00e1ndez-Mill\u00e1n P, V\u00e1zquez-Monteagudo S, Boix E and Prats-Ejarque G. Exploring the RNase A scaffold to combine catalytic and antimicrobial activities. Structural characterization of RNase 3\/1 chimeras (2022) Front. Mol. Biosci<\/em>. 9:964717 https:\/\/doi.org\/10.3389\/fmolb.2022.964717<\/a><\/p>\n

Lu L, Li J, Wei R, Guidi I, Cozzuto L, Ponomarenko J, Prats-Ejarque G and Boix E. Selective cleavage of ncRNA and antiviral activity by RNase2\/EDN in THP1-induced macrophages Cell Mol Life Sci<\/em> (2022) 79:209 https:\/\/link.springer.com\/article\/10.1007\/s00018-022-04229-x<\/a><\/p>\n

Li, J.; Prats-Ejarque, G.; Torrent, M.; Andreu, D.; Brandenburg, K.; Fern\u00e1ndez-Mill\u00e1n, P.; Boix, E<\/strong>. In Vivo Evaluation of ECP Peptide Analogues for the Treatment of Acinetobacter baumannii<\/em> Infection. Biomedicines<\/em> (2022), 10<\/em>, 386. https:\/\/doi.org\/10.3390\/biomedicines10020386<\/a><\/p>\n

Prats-Ejarque G, Lorente H, Villalba C, Anguita R, Lu L, V\u00e1zquez-Monteagudo S, Fern\u00e1ndez-Mill\u00e1n P, Boix E<\/strong>*. Structure-Based Design of an RNase Chimera for Antimicrobial Therapy. Int J Mol Sci<\/em>. (2022) 23(1):95. doi: 10.3390\/ijms23010095.<\/a><\/p>\n

Sand\u00edn D, Valle J, Chaves-Arquero B, Prats-Ejarque G, Larrosa MN, Gonz\u00e1lez-L\u00f3pez JJ, Jim\u00e9nez M\u00c1, Boix E, Andreu D, Torrent M. Rationally Modified Antimicrobial Peptides from the N-Terminal Domain of Human RNase 3 Show Exceptional Serum Stability. J Med Chem<\/em>. (2021) 12;64(15):11472-11482. https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acs.jmedchem.1c00795<\/a><\/p>\n

Li J. and Boix E. Host Defense RNases as Antiviral agents against enveloped single-stranded RNA Viruses. Virulence<\/em> (2021) 12:1, 444-469. https:\/\/doi.org\/10.1080\/21505594.2021.1871823<\/a><\/p>\n

Lu, L.;Wei, R.; Bhakta, S.; Waddell, S.J.; Boix, E.Weighted Gene Co-Expression Network Analysis Identifies Key Modules and Hub Genes Associated with Mycobacterial Infection of Human Macrophages. Antibiotics<\/em> (2021), 10, 97.https:\/\/doi.org\/10.3390\/antibiotics10020097<\/a><\/p>\n

Lu, L., Wei, R., Prats-Ejarque, G. Goetz,\u00a0M, Torrent\u00a0M &\u00a0 Boix E. Human RNase3 immune modulation by catalytic-dependent and independent modes in a macrophage-cell line infection model. Cell. Mol. Life Sci.<\/i> (2021). https:\/\/doi.org\/10.1007\/s00018-020-03695-5<\/a><\/p>\n

Rangel-Mu\u00f1oz N, Suarez-Arnedo A, Anguita R, Prats-Ejarque G, Osma JF,\u00a0 Mu\u00f1oz-Camargo C, Boix E, Cruz JC and Salazar VA Magnetite Nanoparticles Functionalized with RNases against Intracellular Infection of Pseudomonas aeruginosa Pharmaceutics<\/em> 2020, 12(7), 631; https:\/\/doi.org\/10.3390\/pharmaceutics12070631<\/a><\/p>\n

Li J, Fern\u00e1ndez-Mill\u00e1n P, Boix E. Synergism between host defence peptides and antibiotics against bacterial infections. Curr Top Med Chem<\/em>. 2020 Mar 3. doi:10.2174\/1568026620666200303122626.<\/p>\n

Boix E, Acquati F, Leonidas D, Pulido D. Editorial: Role of Ribonucleases in
\nImmune Response Regulation During Infection and Cancer. Front Immunol<\/em>. 2020 Feb
\n19;11:236. doi: 10.3389\/fimmu.2020.00236. eCollection 2020.<\/p>\n

Prats-Ejarque G, Lu L, Salazar VA, Moussaoui M, Boix E. Evolutionary Trends in
\nRNA Base Selectivity Within the RNase A Superfamily. Front Pharmacol.<\/em> 2019 Oct
\n9;10:1170. doi: 10.3389\/fphar.2019.01170.<\/p>\n

Salazar VA, Arranz-Trull\u00e9n J, Prats-Ejarque G, Torrent M, Andreu D, Pulido D,
\nBoix E. Insight into the Antifungal Mechanism of Action of Human RNase N-terminus Derived Peptides. Int J Mol Sci<\/em>. 2019 Sep 14;20(18). pii: E4558. doi:10.3390\/ijms20184558.<\/p>\n

Lu L, Arranz-Trull\u00e9n J, Prats-Ejarque G, Pulido D, Bhakta S, Boix E. Human
\nAntimicrobial RNases Inhibit Intracellular Bacterial Growth and Induce Autophagy
\nin Mycobacteria-Infected Macrophages. Front Immunol<\/em>. 2019 Jul 2;10:1500.https:\/\/doi.org\/10.3389\/fimmu.2019.01500<\/a><\/p>\n

Prats-Ejarque G, Li J, Ait-Ichou F, Lorente H, Boix E. Testing a Human
\nAntimicrobial RNase Chimera Against Bacterial Resistance. Front Microbiol.<\/em> 2019
\nJun 19;10:1357. https:\/\/doi.org\/10.3389\/fmicb.2019.01357<\/a><\/p>\n

Prats-Ejarque G, Blanco JA, Salazar VA, Nogu\u00e9s VM, Moussaoui M, Boix E.
\nCharacterization of an RNase with two catalytic centers. Human RNase6 catalytic
\nand phosphate-binding site arrangement favors the endonuclease cleavage of
\npolymeric substrates. Biochim Biophys Acta Gen Subj<\/em>. 2019 Jan;1863(1):105-117.
\nhttps:\/\/doi.org\/10.1016\/j.bbagen.2018.09.021<\/a><\/p>\n

Lu L, Li J, Moussaoui M and Boix E Immune modulation by human secreted RNases at the extracellular space. Front Immunol<\/em> (2018) 8:1499. 1-17 https:\/\/doi.org\/10.3389\/fimmu.2018.01012<\/a><\/p>\n

Pulido D, Prats-Ejarque G, Villalba C, Albacar M, Moussaoui M, Andreu D,
\nVolkmer R, Torrent M, Boix E. Positional scanning library applied to the human
\neosinophil cationic protein\/RNase3 N-terminus reveals novel and potent
\nanti-biofilm peptides. Eur J Med Chem<\/em>. 2018 May 25;152:590-599.
\nhttps:\/\/doi.org\/10.1016\/j.ejmech.2018.05.012<\/a><\/p>\n

Mu\u00f1oz-Camargo C, Salazar VA, Barrero-Guevara L, Camargo S, Mosquera A, Groot H, Boix E. Unveiling the Multifaceted Mechanisms of Antibacterial Activity of
\nBuforin II and Frenatin 2.3S Peptides from Skin Micro-Organs of the Orinoco Lime
\nTreefrog (Sphaenorhynchus lacteus). Int J Mol Sci<\/em>. 2018 Jul 25;19(8). pii: E2170.
\ndoi: 10.3390\/ijms19082170. PubMed PMID: 30044391; PubMed Central PMCID:
\nPMC6121439.<\/p>\n

Pulido D, Rebollido-Rios R, Valle J, Andreu D, Boix E, Torrent M. Structural
\nsimilarities in the CPC clip motif explain peptide-binding promiscuity between
\nglycosaminoglycans and lipopolysaccharides. J R Soc Interface<\/em>. 2017 Nov;14(136).
\npii: 20170423. doi: 10.1098\/rsif.2017.0423. PubMed PMID: 29187635; PubMed CentralPMCID: PMC5721150.<\/p>\n

Arranz-Trull\u00e9n J, Lu L, Pulido D, Bhakta S, Boix E. Host Antimicrobial
\nPeptides: The Promise of New Treatment Strategies against Tuberculosis. Front<\/em>
\nImmunol<\/em>. 2017 Nov 7;8:1499. doi: 10.3389\/fimmu.2017.01499. eCollection 2017.
\nReview. PubMed PMID: 29163551; PubMed Central PMCID: PMC5681943.<\/p>\n

Pulido D, Garcia-Mayoral MF, Moussaoui M, Vel\u00e1zquez D, Torrent M, Bruix M,
\nBoix E. Structural basis for endotoxin neutralization by the eosinophil cationic
\nprotein. FEBS J. 2016<\/em> Nov;283(22):4176-4191. doi: 10.1111\/febs.13915. Epub 2016
\nOct 31. PubMed PMID: 27696685.<\/p>\n

Pulido D, Prats-Ejarque G, Villalba C, Albacar M, Gonz\u00e1lez-L\u00f3pez JJ, Torrent
\nM, Moussaoui M, Boix E. A Novel RNase 3\/ECP Peptide for Pseudomonas aeruginosa Biofilm Eradication That Combines Antimicrobial, Lipopolysaccharide Binding, and Cell-Agglutinating Activities. Antimicrob Agents Chemother<\/em>. 2016 Sep23;60(10):6313-25. doi: 10.1128\/AAC.00830-16. Print 2016 Oct. PubMed PMID:
\n27527084; PubMed Central PMCID: PMC5038260.<\/p>\n

Salazar VA, Arranz-Trull\u00e9n J, Navarro S, Blanco JA, S\u00e1nchez D, Moussaoui M,
\nBoix E. Exploring the mechanisms of action of human secretory RNase 3 and RNase 7 against Candida albicans. Microbiologyopen<\/em>. 2016 Oct;5(5):830-845. doi:10.1002\/mbo3.373. Epub 2016 Jun 8. PubMed PMID: 27277554; PubMed Central PMCID:PMC5061719.<\/p>\n

Pulido D, Arranz-Trull\u00e9n J, Prats-Ejarque G, Vel\u00e1zquez D, Torrent M, Moussaoui
\nM, Boix E. Insights into the Antimicrobial Mechanism of Action of Human RNase6:
\nStructural Determinants for Bacterial Cell Agglutination and Membrane Permeation.
\nInt J Mol Sci. 2016 Apr 13;17(4):552. doi: 10.3390\/ijms17040552. PubMed PMID:
\n27089320; PubMed Central PMCID: PMC4849008.<\/p>\n

Prats-Ejarque G, Arranz-Trull\u00e9n J, Blanco JA, Pulido D, Nogu\u00e9s MV, Moussaoui
\nM, Boix E. The first crystal structure of human RNase 6 reveals a novel
\nsubstrate-binding and cleavage site arrangement. Biochem J. 2016 Jun
\n1;473(11):1523-36. doi: 10.1042\/BCJ20160245. Epub 2016 Mar 24. PubMed PMID:
\n27013146; PubMed Central PMCID: PMC4888456.<\/p>\n

Salazar VA, Rubin J, Moussaoui M, Pulido D, Nogu\u00e9s MV, Venge P, Boix E.
\nProtein post-translational modification in host defense: the antimicrobial
\nmechanism of action of human eosinophil cationic protein native forms. FEBS J.
\n2014 Dec;281(24):5432-46. doi: 10.1111\/febs.13082. Epub 2014 Oct 20. PubMed PMID:
\n25271100.<\/p>\n

Pulido D, Moussaoui M, Nogu\u00e9s MV, Torrent M, Boix E. Towards the rational
\ndesign of antimicrobial proteins: single point mutations can switch on
\nbactericidal and agglutinating activities on the RNase A superfamily lineage.
\nFEBS J. 2013 Nov;280(22):5841-52. doi: 10.1111\/febs.12506. Epub 2013 Oct 8.
\nPubMed PMID: 23992292.<\/p>\n

Torrent M, Pulido D, Valle J, Nogu\u00e9s MV, Andreu D, Boix E. Ribonucleases as a
\nhost-defence family: evidence of evolutionarily conserved antimicrobial activity
\nat the N-terminus. Biochem J. 2013 Nov 15;456(1):99-108. doi: 10.1042\/BJ20130123.
\nPubMed PMID: 23962023.<\/p>\n

Pulido D, Torrent M, Andreu D, Nogu\u00e9s MV, Boix E. Two human host defense
\nribonucleases against mycobacteria, the eosinophil cationic protein (RNase 3) and
\nRNase 7. Antimicrob Agents Chemother. 2013 Aug;57(8):3797-805. doi:
\n10.1128\/AAC.00428-13. Epub 2013 May 28. PubMed PMID: 23716047; PubMed Central
\nPMCID: PMC3719706.<\/p>\n

Boix E, Blanco JA, Nogu\u00e9s MV, Moussaoui M. Nucleotide binding architecture
\nfor secreted cytotoxic endoribonucleases. Biochimie. 2013 Jun;95(6):1087-97. doi:
\n10.1016\/j.biochi.2012.12.015. Epub 2012 Dec 26. Review. PubMed PMID: 23274129.<\/p>\n

Torrent M, Pulido D, Nogu\u00e9s MV, Boix E. Exploring new biological functions of
\namyloids: bacteria cell agglutination mediated by host protein aggregation. PLoS
\nPathog. 2012;8(11):e1003005. doi: 10.1371\/journal.ppat.1003005. Epub 2012 Nov 1.
\nPubMed PMID: 23133388; PubMed Central PMCID: PMC3486885.<\/p>\n

Garc\u00eda-Mayoral MF, Canales A, D\u00edaz D, L\u00f3pez-Prados J, Moussaoui M, de Paz JL,
\nAngulo J, Nieto PM, Jim\u00e9nez-Barbero J, Boix E, Bruix M. Insights into the
\nglycosaminoglycan-mediated cytotoxic mechanism of eosinophil cationic protein
\nrevealed by NMR. ACS Chem Biol. 2013 Jan 18;8(1):144-51. doi: 10.1021\/cb300386v.
\nEpub 2012 Oct 9. PubMed PMID: 23025322.<\/p>\n

Boix E, Salazar VA, Torrent M, Pulido D, Nogu\u00e9s MV, Moussaoui M. Structural
\ndeterminants of the eosinophil cationic protein antimicrobial activity. Biol
\nChem. 2012 Aug;393(8):801-15. doi: 10.1515\/hsz-2012-0160. Review. PubMed PMID:
\n22944682.<\/p>\n

Torrent M, Nogu\u00e9s MV, Andreu D, Boix E. The “CPC clip motif”: a conserved
\nstructural signature for heparin-binding proteins. PLoS One. 2012;7(8):e42692.
\ndoi: 10.1371\/journal.pone.0042692. Epub 2012 Aug 6. PubMed PMID: 22880084; PubMed
\nCentral PMCID: PMC3412806.<\/p>\n

Torrent M, Nogu\u00e9s MV, Boix E. Discovering new in silico tools for
\nantimicrobial peptide prediction. Curr Drug Targets. 2012 Aug;13(9):1148-57.
\nPubMed PMID: 22664076.<\/p>\n

Boix E. Editorial: novel strategies for the design of therapeutic
\nantimicrobial peptides. Curr Drug Targets. 2012 Aug;13(9):1119-20. PubMed PMID:
\n22664070.<\/p>\n

Boix E, Pulido D, Moussaoui M, Nogu\u00e9s MV, Russi S. The sulfate-binding site
\nstructure of the human eosinophil cationic protein as revealed by a new crystal
\nform. J Struct Biol. 2012 Jul;179(1):1-9. doi: 10.1016\/j.jsb.2012.04.023. Epub
\n2012 May 9. PubMed PMID: 22579681.<\/p>\n

Pulido D, Nogu\u00e9s MV, Boix E, Torrent M. Lipopolysaccharide neutralization by
\nantimicrobial peptides: a gambit in the innate host defense strategy. J Innate
\nImmun. 2012;4(4):327-36. doi: 10.1159\/000336713. Epub 2012 Mar 21. Review. PubMed
\nPMID: 22441679.<\/p>\n

Pulido D, Moussaoui M, Andreu D, Nogu\u00e9s MV, Torrent M, Boix E. Antimicrobial
\naction and cell agglutination by the eosinophil cationic protein are modulated by
\nthe cell wall lipopolysaccharide structure. Antimicrob Agents Chemother. 2012
\nMay;56(5):2378-85. doi: 10.1128\/AAC.06107-11. Epub 2012 Feb 13. PubMed PMID:
\n22330910; PubMed Central PMCID: PMC3346588.<\/p>\n

Torrent M, Di Tommaso P, Pulido D, Nogu\u00e9s MV, Notredame C, Boix E, Andreu D.
\nAMPA: an automated web server for prediction of protein antimicrobial regions.
\nBioinformatics. 2012 Jan 1;28(1):130-1. doi: 10.1093\/bioinformatics\/btr604. Epub
\n2011 Nov 3. PubMed PMID: 22053077.<\/p>\n","protected":false},"excerpt":{"rendered":"

Selected publications Anguita R, Li J, Boix E and Prats-Ejarque G A Novel Method to Monitor the Evolution of Antimicrobial Resistance in Acinetobacter baumannii Biofilms. IJMS. 2026; 27, 1512 Anguita R, Ali Y, Becknell B and Boix E* Ribonucleases of Mice and Men: Unveiling the Roles of the RNase A Superfamily in Host Defence J […]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-76","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/webs.uab.cat\/hrnases\/en\/wp-json\/wp\/v2\/pages\/76","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/webs.uab.cat\/hrnases\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/webs.uab.cat\/hrnases\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/webs.uab.cat\/hrnases\/en\/wp-json\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/webs.uab.cat\/hrnases\/en\/wp-json\/wp\/v2\/comments?post=76"}],"version-history":[{"count":10,"href":"https:\/\/webs.uab.cat\/hrnases\/en\/wp-json\/wp\/v2\/pages\/76\/revisions"}],"predecessor-version":[{"id":280,"href":"https:\/\/webs.uab.cat\/hrnases\/en\/wp-json\/wp\/v2\/pages\/76\/revisions\/280"}],"wp:attachment":[{"href":"https:\/\/webs.uab.cat\/hrnases\/en\/wp-json\/wp\/v2\/media?parent=76"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}