{"id":187,"date":"2025-03-17T13:26:48","date_gmt":"2025-03-17T11:26:48","guid":{"rendered":"https:\/\/webs.uab.cat\/rna-rnase\/?page_id=187"},"modified":"2025-03-24T17:29:10","modified_gmt":"2025-03-24T15:29:10","slug":"spring-workshop","status":"publish","type":"page","link":"https:\/\/webs.uab.cat\/rna-rnase\/spring-workshop\/","title":{"rendered":"Spring Workshop"},"content":{"rendered":"\n<h1 class=\"wp-block-heading alignfull has-text-align-right has-verd-tropic-color has-text-color has-link-color has-larger-font-size wp-elements-4d641cd255fdef41779417a13a6b31d7\">7th April 2025<\/h1>\n\n\n\n<div class=\"wp-block-columns alignfull is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column has-verd-tropic-transparent-background-color has-background is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"has-text-align-center has-large-font-size\">09:20-10:00<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p class=\"has-text-align-center has-larger-font-size\"><strong>Dr. Roeland Boer<\/strong><\/p>\n<\/blockquote>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<p class=\"has-text-align-center\">XALOC Beamline,<\/p>\n\n\n\n<p class=\"has-text-align-center\">ALBA Synchrotron, Spain<\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-blanc-coco-color has-negre-eben-background-color has-text-color has-background has-link-color wp-elements-b301219b6c56826cda3fc31fd5f48e62\"><strong><a href=\"https:\/\/www.cells.es\/en\/instruments\/beamlines\/bl13-xaloc\">BL13 &#8211; XALOC &#8211; ALBA Synchrotron<\/a><\/strong><\/p>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\">More details<\/a><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column has-verd-tropic-transparent-background-color has-background is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"has-text-align-center has-large-font-size\">10:00-10:40<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p class=\"has-text-align-center has-larger-font-size\"><strong>Dr. Lin Huang<\/strong><\/p>\n<\/blockquote>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<p class=\"has-text-align-center\">Group Leader Guangdong-Hong Kong Joint Laboratory for RNA Medicine,&nbsp;Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, China<\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-blanc-coco-color has-negre-eben-background-color has-text-color has-background has-link-color wp-elements-08caf4da67a537f415dc170cafa758c6\"><a href=\"https:\/\/lab.rjmart.cn\/10109\/NASRG\/\"><strong>Nucleic Acid Structure Research Group<\/strong><\/a><\/p>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/webs.uab.cat\/rna-rnase\/spring-workshop\/#Huang_Lin\">More details<\/a><\/div>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column has-verd-tropic-transparent-background-color has-background is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"has-text-align-center has-large-font-size\">10:40-11:20<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p class=\"has-text-align-center has-larger-font-size\"><strong>Dr.Ana Joaquina P\u00e9rez-Berna<\/strong><\/p>\n<\/blockquote>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-center is-layout-flex wp-container-core-group-is-layout-4b2eccd6 wp-block-group-is-layout-flex\">\n<p class=\"has-text-align-center\">MISTRAL Beamline Scientist,<\/p>\n\n\n\n<p class=\"has-text-align-center\">ALBA Synchrotron, Spain<\/p>\n<\/div>\n\n\n\n<p class=\"has-text-align-center has-blanc-coco-color has-negre-eben-background-color has-text-color has-background has-link-color wp-elements-f0808bf2e10d46b7883ffdffbc8bd585\"><a href=\"https:\/\/www.cells.es\/en\/instruments\/beamlines\/bl01-miras\"><\/a><a href=\"https:\/\/www.cells.es\/en\/instruments\/beamlines\/bl09-mistral\"><strong>BL09 &#8211; MISTRAL &#8211; ALBA Synchrotron<\/strong><\/a><\/p>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/webs.uab.cat\/rna-rnase\/spring-workshop\/#Ana_Joaquina\">More details<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator alignfull has-alpha-channel-opacity\" \/>\n\n\n\n<div class=\"wp-block-columns alignfull are-vertically-aligned-center is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:80%\">\n<blockquote class=\"wp-block-quote alignwide is-layout-flow wp-block-quote-is-layout-flow\">\n<h1 class=\"wp-block-heading alignwide has-verd-tropic-color has-gris-pubilla-background-color has-text-color has-background has-link-color has-larger-font-size wp-elements-4efa39ed3909e1f45e55078dec5c93af\">Time: 09:20-10:00_<strong><strong><strong>Dr. Roeland Boer<\/strong><\/strong><\/strong><\/h1>\n<\/blockquote>\n\n\n\n<blockquote class=\"wp-block-quote alignwide is-layout-flow wp-block-quote-is-layout-flow\">\n<h1 class=\"wp-block-heading alignwide has-verd-tropic-color has-gris-pubilla-background-color has-text-color has-background has-link-color has-larger-font-size wp-elements-0d50c5f8cf4162d9a37b37cb240714ad\" id=\"Huang_Lin\">Time: 10:00-10:40_<strong><strong>Dr. Lin Huang<\/strong><\/strong><\/h1>\n<\/blockquote>\n\n\n\n<details class=\"wp-block-mamaduka-toggles alignwide wp-block-toggles has-larger-font-size\"><summary><strong><strong>Enhancing RNA Crystallization and Resolution via Some general strategies<\/strong><\/strong><\/summary><div class=\"wp-block-toggles__content\">\n<ul class=\"wp-block-list\">\n<li class=\"has-normal-font-size\">&nbsp;X-ray crystallography is a fundamental technique for determining RNA structures at atomic resolution. However, obtaining high-resolution RNA crystals remains a significant challenge. Here, we present a novel strategy to improve the resolution of RNA crystals by selectively substituting Watson\u2013Crick base pairs with GU pairs within RNA sequences. Our approach has successfully yielded high-resolution structures for eight unique RNA crystals. Notably, six cases demonstrated marked resolution enhancement upon GC\/AU to GU base pair substitution, with two instances achieving high-resolution structures from initially poor data. Interestingly, reverting GU to GC base pairs also improved resolution in one case. This method facilitated the first structural determinations of the LINE-1 and OR4K15 ribozymes, the 2\u2032-deoxyguanosine-III riboswitch, and the Broccoli RNA aptamer. We show that placing GU base pairs within the first 5\u2032 helical stem or in one peripheral stem of any given RNA species is sufficient. These findings provide a simple and effective approach for designing or selecting RNA sequences to facilitate high-resolution structure determination.&nbsp;<\/li>\n\n\n\n<li class=\"has-normal-font-size\">Furthermore, I will also present additional methods developed in my laboratory that aid in RNA crystallization.&nbsp;<\/li>\n<\/ul>\n<\/div><\/details>\n\n\n\n<blockquote class=\"wp-block-quote alignwide is-layout-flow wp-block-quote-is-layout-flow\">\n<h1 class=\"wp-block-heading alignwide has-verd-tropic-color has-gris-pubilla-background-color has-text-color has-background has-link-color has-larger-font-size wp-elements-ca5cad05949a4bbc182b7d0581532f03\" id=\"Ana_Joaquina\">Time: 10:40-11:20_<strong>Dr.Ana Joaquina P\u00e9rez-Berna<\/strong><\/h1>\n<\/blockquote>\n\n\n\n<details class=\"wp-block-mamaduka-toggles alignwide wp-block-toggles has-larger-font-size\"><summary><strong>Correlative imaging of biological cells by cryo-soft-Xray-tomography<\/strong><\/summary><div class=\"wp-block-toggles__content\">\n<div class=\"wp-block-columns alignfull is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:50%\">\n<ul class=\"wp-block-list has-normal-font-size\">\n<li class=\"has-normal-font-size\">Cryo soft X-ray tomography (SXT) of whole hydrated cells in the water window energy range can provide relevant structural information of complex cellular phenomena with chemical sensitivity at spatial resolutions of 30 nm half pitch<em> [1]<\/em>.\u00a0Functional studies can be achieved by correlating this information with cryo visible light fluorescence microscopy in 2D or 3D <em>[2, 3]<\/em>, as well as with 3D cryo hard X-ray fluorescence to localize and quantify specific molecules. The chemical insight into cellular aspects could be also correlated the cellular 3D structural maps with infrared microscopy, using synchrotron-based infrared microscopy (MIRAS-beamline) <em>[4]<\/em>. In addition, spectroscopic imaging can also be used to understand biomineralization processes <em>[5]<\/em>. Examples of 3D correlative cryo X-ray imaging research will be presented.\u00a0<\/li>\n<\/ul>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:50%\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"592\" src=\"https:\/\/webs.uab.cat\/rna-rnase\/wp-content\/uploads\/sites\/602\/2025\/03\/Picture-1.gif\" alt=\"\" class=\"wp-image-258\" \/><\/figure>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p class=\"has-small-font-size\">&nbsp;<strong>REFERENCES&nbsp;<\/strong><\/p>\n\n\n\n<p class=\"has-small-font-size\">[1] Schneider G. et al. Nat. Methods 7: 985-987 (2010)&nbsp;<\/p>\n\n\n\n<p class=\"has-small-font-size\">[2] P\u00e9rez-Bern\u00e1 A.J. et al. ACS Nano 10, 6597-6611 (2016)&nbsp;<\/p>\n\n\n\n<p class=\"has-small-font-size\">[3] P\u00e9rez-Bern\u00e1 A.J. et al. ACS Nano ACSnano, 17(22), pp. 22708\u201322721 (2023)&nbsp;<\/p>\n\n\n\n<p class=\"has-small-font-size\">[4] P\u00e9rez-Bern\u00e1 A.J. et al. Acta Crystallographica Section D. ActaCryst.(2021). Nov. 2111\/58(12)10&nbsp;<\/p>\n\n\n\n<p class=\"has-small-font-size\">[5] Kahil K et al. PNAS 117 (49), 30957-30965 (2020).&nbsp;<\/p>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<ul class=\"wp-block-list has-normal-font-size\">\n<li>Our results also constitute a proof of concept for the use of cryo-SXT as a platform that enables determining the potential impact of candidate compounds on the ultrastructure of the cell that may assist drug development at a preclinical level.\u00a0<\/li>\n<\/ul>\n<\/div><\/details>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center has-verd-tropic-transparent-background-color has-background is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:20%\">\n<figure class=\"wp-block-pullquote\"><blockquote><p><strong>Teams link for the event<\/strong><\/p><cite>7th April 2025<\/cite><\/blockquote><\/figure>\n\n\n\n<div class=\"wp-block-buttons alignwide is-content-justification-right is-layout-flex wp-container-core-buttons-is-layout-765c4724 wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-100\"><a class=\"wp-block-button__link has-text-align-center wp-element-button\" href=\"https:\/\/teams.microsoft.com\/l\/meetup-join\/19%3ameeting_YzNiOGM1ZmUtNTVhZS00M2NkLWJlN2EtYzhlMmQ3YWZjMmM0%40thread.v2\/0?context=%7b%22Tid%22%3a%226b514c29-2391-4831-b774-84f35c45bf01%22%2c%22Oid%22%3a%2207ca3a01-7ee9-4ddc-8701-b7f7dc5522f3%22%7d\">teams<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>7th April 2025 09:20-10:00 Dr. Roeland Boer XALOC Beamline, ALBA Synchrotron, Spain BL13 &#8211; XALOC &#8211; ALBA Synchrotron 10:00-10:40 Dr. Lin Huang Group Leader Guangdong-Hong Kong Joint Laboratory for RNA Medicine,&nbsp;Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, China Nucleic Acid Structure Research Group 10:40-11:20 Dr.Ana Joaquina P\u00e9rez-Berna MISTRAL Beamline Scientist, ALBA Synchrotron, [&hellip;]<\/p>\n","protected":false},"author":2988,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-187","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/webs.uab.cat\/rna-rnase\/wp-json\/wp\/v2\/pages\/187","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/webs.uab.cat\/rna-rnase\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/webs.uab.cat\/rna-rnase\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/webs.uab.cat\/rna-rnase\/wp-json\/wp\/v2\/users\/2988"}],"replies":[{"embeddable":true,"href":"https:\/\/webs.uab.cat\/rna-rnase\/wp-json\/wp\/v2\/comments?post=187"}],"version-history":[{"count":100,"href":"https:\/\/webs.uab.cat\/rna-rnase\/wp-json\/wp\/v2\/pages\/187\/revisions"}],"predecessor-version":[{"id":326,"href":"https:\/\/webs.uab.cat\/rna-rnase\/wp-json\/wp\/v2\/pages\/187\/revisions\/326"}],"wp:attachment":[{"href":"https:\/\/webs.uab.cat\/rna-rnase\/wp-json\/wp\/v2\/media?parent=187"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}