UAT offers the following services:
– Generation of transgenic mice and rats by pronuclear microinjection of DNA transgenes and BACs.
– Generation of KO/KI mouse models by gene targeting:
– Obtention of recombinant ESC by homologous recombination.
– Injection of ESC clones (internal or external sources) into blastocysts.
– Generation of KO/KI mouse and rat models by CRISPR/Cas9 system.
– Rederivation of mouse/rat models by embryo transfer or in vitro fertilization (IVF).
– Cryopreservation of mouse/rat embryos, sperm and ovary.
– Recovery of mouse/rat models from frozen embryos, sperm or ovary.
– Analysis of genotype by PCR and Southern blot.
– Advice on the design and generation of genetically modified mouse and rat models, as well as on the establishment and management of transgenic mouse/rat colonies.
