They are given by Dr. Maria Martínez García and Dr. Carol Ripollés Ávila, in the laboratory V0-160. Attendance of the three sessions (Wednesday 22nd, Thursday 23rd and Friday 24th) is required. Attendance is limited to 26 people per group. Different rapid methods are assayed and compared.
Wednesday, 22nd November · 9:15 a.m.-12 noon
• Sample preparation and plating automated procedures: dilutors DiluFlow Pro, Smart Dilutor W, Diluwel and Dilumat, homogenizers BagMixer SW, Pulsifier, Masticator and Smasher, serial dilution systems Dilucup-Dilugent Shaker and Serial Diluter; spiral platers easySpiral Dilute and Eddy Jet 2W
• Rapid viable cell count methods: Vitroids and Lenticule discs (certified reference microorganisms); Petrifilm (plates and reader Advanced), DryPlates, Compact-Dry (plates and reader); Colilert-18, Enterolert-DW, Pseudalert and Quanti-Tray; Milliflex Quantum; SimPlate; colony counters Scan 1200, SphereFlash and Quantica 500
• Method for enumeration by miniaturized MPN: TEMPO AC and EC
Thursday, 23rd November – Thanksgiving day · 9:15-11:55 a.m.
• Environmental control procedures: RODAC, Count-Tact and Lock&Block plates; contact slides; Quick Swab; samplers Spin Air, MicroBio, Airwel and AIR IDEAL 3P
• Chromogenic culture media: SALMA One Day, ALOA, ChromID Coli, ChromID EHEC, Baird-Parker RPF agar, Brilliance Salmonella agar (Oxoid Salmonella Precis method), Brilliance Listeria agar (Oxoid Listeria Precis method), Brilliance Staph 24 agar, Brilliance coliform agar, RAPID’Salmonella, RAPID’E.coli 2, RAPID’L.mono, IRIS Salmonella agar, COMPASS Listeria agar, Chromocult coliform agar
• Diagnostic kits: API (kit and reader), RapID ONE, Microbact, rhamnose test, EnteroPluri-Test, HACCP System Plus
• Other environmental control procedures:
– luminescence: UltraSnap, AquaSnap, MicroSnap
• Immunological detection methods:
– ELFA: VIDAS SPT
– immunoprecipitation: 1-2 Test for Salmonella
– lateral immunomigration: Singlepath, VIP Gold, Reveal 2.0 for Salmonella
• Method for enumeration by miniaturized MPN: reading of TEMPO AC and EC
Friday, 24th November · 9-11 a.m.
• Diagnostic kits: O·B·I·S·, ID membranes
• Other environmental control procedures:
– luminescence: N-Light Listeria monocytogenes, luminometers Clean-Trace, AccuPoint Advanced, MVP ICON and EnSURE Touch
– colorimetry: Contam Swab, FLASH, Clean Test, InSite, AllerSnap
– others: BioFinder
• Immunological detection methods:
– confirmation by latex agglutination: Oxoid latex test
– lateral immunomigration: Reveal Q+ for aflatoxins and for DON (kits and reader Raptor), Reveal 3D for allergens (milk), AllerFlow gluten, DipSensor and Extenso for antibiotics
• Molecular biology (methods other than PCR): Molecular Detection System (MDS)
• Reading: rapid viable cell count methods, environmental control procedures, chromogenic culture media, diagnostic kits, immunological detection methods