They are given by Dr. Maria Martínez García and Dr. Carol Ripollés Ávila, in the laboratory V0-160. Attendance of the three sessions (Wednesday 25th, Thursday 26th and Friday 27th) is required. Attendance is limited to 30 people. Different rapid methods are assayed and compared.
Wednesday, 25th November · 9:15 a.m.-12:05 p.m.
• Sample preparation and plating automated procedures: dilutors DiluFlow Elite, Diluwel and Dilumat, homogenizers BagMixer SW, Pulsifier, Masticator SWAP and Smasher, serial dilution systems Dilucup-Dilugent Shaker and Serial Diluter; spiral plater easySpiral Dilute
• Rapid viable cell count methods: Vitroids and Lenticule discs (certified reference microorganisms); Petrifilm (plates and reader Advanced), DryPlates, Compact-Dry (plates and reader), MicroFast; Rapid YM agar, Quanti-P/A Clostricult; Colilert-18, Pseudalert and Quanti-Tray; EZ-Fluo; SimPlate; colony counter Scan 3000 Ai
• Method for enumeration by miniaturized MPN: TEMPO AC and EC
Thursday, 26th November – Thanksgiving day · 9:15-11:55 a.m.
• Environmental control procedures: RODAC, Count-Tact and Lock&Block plates; contact slides; Quick Swab; samplers MicroBio, Airwel, AIR IDEAL 3P and SAS Super DUO 360
• Chromogenic culture media: ALOA, ChromID Coli, ChromID EHEC, Baird-Parker RPF agar, Brilliance Salmonella agar (Oxoid Salmonella Precis method), Brilliance Listeria agar (Oxoid Listeria Precis method), Brilliance Staph 24 agar, Brilliance coliform agar, RAPID’Salmonella, RAPID’E.coli 2, RAPID’L.mono, IRIS Salmonella agar, COMPASS Listeria agar, Chromocult coliform agar, CondaChrome agar for Salmonella
• Diagnostic kits: Rapidec Lmono, RapID ONE, Microbact, rhamnose test, EnteroPluri-Test, HACCP System Plus
• Other environmental control procedures:
– luminescence: UltraSnap, AquaSnap, MicroSnap
– colorimetry: Food Swab
• Immunological detection methods:
– ELFA: VIDAS KUBE
– lateral immunomigration: Singlepath, VIP Gold, Reveal 2.0 for Salmonella
• Method for enumeration by miniaturized MPN: reading of TEMPO AC and EC
Friday, 27th November · 9-11 a.m.
• Diagnostic kits: O·B·I·S·, ID membranes
• Other environmental control procedures:
– luminescence: N-Light Listeria monocytogenes, ATP-FLOW Surface, luminometers Clean-Trace, MVP ICON, PhosReader 2 and EnSURE Touch
– colorimetry: FLASH, InSite, AllerSnap, PROTEIN-FLOW
– others: MicroDetector, TBF 300, BioFinder
• Immunological detection methods:
– confirmation by latex agglutination: Oxoid latex test
– lateral immunomigration: PrecisCheck Listeria, Reveal Q+ for aflatoxins and for DON (kits and reader Raptor), Reveal 3D for allergens (milk), Symmetric Green for aflatoxins and for DON and Free Test for allergens (beta-lactoglobulin, casein, gluten) (kits and reader 3PR Mini), AllerFlow gluten, DipSensor and Extenso for antibiotics
• Molecular biology (methods other than PCR): Molecular Detection System (MDS), AMP-Loop-mediated Isothermal Amplification (AMP-LAMP)
• Reading: rapid viable cell count methods, environmental control procedures, chromogenic culture media, diagnostic kits, immunological detection methods