9-9:45 a.m. Handout material delivery

9:45-10:15 p.m. Official opening of the workshop

10:15-11:35 a.m. Opening lecture by Dr. José Juan Rodríguez Jerez (Universitat Autònoma de Barcelona, Bellaterra –Cerdanyola del Vallès–, Barcelona)

 Overview on rapid methods, automation and miniaturization in microbiology

Sampling of solid and liquid foods, surfaces, and air

Miniaturization: microtiter plate, most probable number (MPN), viable cell counts. Diagnostic kits

Methods for viable cell counts: hydrophobic grid, spiral plating, flow cytometry, direct epifluorescence filter technique (DEFT), turbidity

Methods for viable cell counts, based on electric impedance and conductance

Methods for viable cell counts, based on ATP – bioluminiscence and colorimetry

Immunological methods for microbial and toxin detection and identification: immunomagnetic separation, ELISA and ELFA, reversed passive latex agglutination, lateral immunomigration, immunoprecipitation

Molecular biology methods for microbial detection and identification: hybridization (DNA and rRNA probes), polymerase chain reaction (PCR), DNA and rRNA characterization (fingerprinting, riboprinting), sequencing, proteomics

11:35-11:50 a.m. Breakfast

11:50 a.m.-1:10 p.m. Dr. Armand Sánchez Bonastre (Universitat Autònoma de Barcelona, Bellaterra –Cerdanyola del Vallès–, Barcelona):

 Polymerase chain reaction (PCR) and massive genome sequencing applied to food safety

1:10-2:40 p.m. Lunch

2:40-5:35 p.m. Microbiology companies · exhibition (Yersin group):

 Solutions for automation of food and beverage microbiological control: from sampling to result interpretation (Avantor – VWR International Eurolab)

 Food safety: molecular solutions for allergens, fraud and emerging pathogens (Deltalab member of SCGP)

 Automatic colony counters: accuracy, repeatability and data integrity guaranteed (Interscience)

 Speeding up and automation in culture media preparation: from dehydrated medium until ready-to-use medium (Scharlab)

5:35-5:45 p.m. Break

5:45-6:30 p.m. Mr. Alfredo Corujo Fernández (Trouw Nutrition España, Tres Cantos, Madrid):

 Implementation of a flexible and rapid molecular tool for Salmonella spp. control programme in aviculture

6:30 p.m. Merienda

Wednesday, 27th November