9-9:45 a.m. Handout material delivery

9:45-10:15 p.m. Official opening of the workshop

10:15-11:35 a.m. Opening lecture by Dr. José Juan Rodríguez Jerez (Universitat Autònoma de Barcelona, Bellaterra –Cerdanyola del Vallès–, Barcelona)

 Overview on rapid methods, automation and miniaturization in microbiology

Sampling of solid and liquid foods, surfaces, and air

Miniaturization: microtiter plate, most probable number (MPN), viable cell counts. Diagnostic kits

Methods for viable cell counts: hydrophobic grid, spiral plating, flow cytometry, direct epifluorescence filter technique (DEFT), turbidity

Methods for viable cell counts, based on electric impedance and conductance

Methods for viable cell counts, based on ATP – bioluminiscence and colorimetry

Immunological methods for microbial and toxin detection and identification: immunomagnetic separation, ELISA and ELFA, reversed passive latex agglutination, lateral immunomigration, immunoprecipitation

Molecular biology methods for microbial detection and identification: hybridization (DNA and rRNA probes), polymerase chain reaction (PCR), DNA and rRNA characterization (fingerprinting, riboprinting), sequencing, proteomics

11:35-11:50 a.m. Breakfast

11:50 a.m.-1:10 p.m. Dr. Armand Sánchez Bonastre (Universitat Autònoma de Barcelona, Bellaterra –Cerdanyola del Vallès–, Barcelona):

 Polymerase chain reaction (PCR) and massive genome sequencing applied to food safety

1:10-2:40 p.m. Lunch

2:40-5:35 p.m. Microbiology companies · exhibition (Yersin group):

 MALDI Biotyper and IR Biotyper: rapid and efficient technologies for microbial identification and typing in food environment (Bruker Española)

 Condagene: quick pathogen detection by qPCR​ (Condalab)

 Food safety: molecular solutions for allergens, fraud and emerging pathogens (Grupo Deltalab)

 Work optimization in microbiology laboratories: from sample preparation to analysis (Interscience)

5:35-5:45 p.m. Break

5:45-6:30 p.m. Dr. Anna Pinar Méndez (Aigües de Barcelona, Empresa Metropolitana de Gestió del Cicle Integral de l’Aigua, Barcelona):

 Beyond legislation: comparison between culture and molecular methods for drinking water quality assessment

6:30 p.m. Merienda

Wednesday, 22nd November