9-9:45 a.m. Handout material delivery
9:45-10:15 p.m. Official opening of the workshop
10:15-11:35 a.m. Opening lecture by Dr. José Juan Rodríguez Jerez (Universitat Autònoma de Barcelona, Bellaterra –Cerdanyola del Vallès–, Barcelona)
• Overview on rapid methods, automation and miniaturization in microbiology
– Sampling of solid and liquid foods, surfaces, and air
– Miniaturization: microtiter plate, most probable number (MPN), viable cell counts. Diagnostic kits
– Methods for viable cell counts: hydrophobic grid, spiral plating, flow cytometry, direct epifluorescence filter technique (DEFT), turbidity
– Methods for viable cell counts, based on electric impedance and conductance
– Methods for viable cell counts, based on ATP – bioluminiscence and colorimetry
– Immunological methods for microbial and toxin detection and identification: immunomagnetic separation, ELISA and ELFA, reversed passive latex agglutination, lateral immunomigration, immunoprecipitation
– Molecular biology methods for microbial detection and identification: hybridization (DNA and rRNA probes), polymerase chain reaction (PCR), DNA and rRNA characterization (fingerprinting, riboprinting), sequencing, proteomics
11:35-11:50 a.m. Breakfast
11:50 a.m.-1:10 p.m. Dr. Armand Sánchez Bonastre (Universitat Autònoma de Barcelona, Bellaterra –Cerdanyola del Vallès–, Barcelona):
• Polymerase chain reaction (PCR) and massive genome sequencing applied to food safety
1:10-2:40 p.m. Lunch
2:40-5:35 p.m. Microbiology companies · exhibition (Yersin group):
• MALDI Biotyper and IR Biotyper: rapid and efficient technologies for microbial identification and typing in food environment (Bruker Española)
• Condagene: quick pathogen detection by qPCR (Condalab)
• Food safety: molecular solutions for allergens, fraud and emerging pathogens (Grupo Deltalab)
• Work optimization in microbiology laboratories: from sample preparation to analysis (Interscience)
5:35-5:45 p.m. Break
5:45-6:30 p.m. Dr. Anna Pinar Méndez (Aigües de Barcelona, Empresa Metropolitana de Gestió del Cicle Integral de l’Aigua, Barcelona):
• Beyond legislation: comparison between culture and molecular methods for drinking water quality assessment
6:30 p.m. Merienda